Kalyan C. Kondapalli, Ph.D.

Associate Professor of Biology
College of Arts, Sciences, and Letters
Natural Sciences
313-593-5027

Teaching Areas:

Biological Sciences

Research Areas:

Cellular and Molecular Physiology

Biography and Education

Kalyan C. Kondapalli joined the Department of Natural Sciences in Fall 2014. Prior to joining the faculty at the University of Michigan-Dearborn, he was a postdoctoral fellow at the Johns Hopkins University’s School of Medicine. Dr. Kondapalli has a unique cross-disciplinary background. While his graduate training was in protein biochemistry and investigative approaches were mainly biophysical, his postdoctoral work complemented this with specific training in cell biology and cellular physiology. He used the opportunities to gain expertise in a number of diverse techniques, while working towards a common goal: rigorous mechanistic characterization of cellular and molecular ion regulation that underlies health and disease. During all those years he mentored several undergraduate and graduate students, and also published with them as co-authors. As member and leader of Johns Hopkins Postdoctoral Association, one of the largest postdoctoral associations in the country, he co-developed an undergraduate training program that utilized the “teacher scholar model”. He found this combination of a rigorous laboratory research experience with a mentored teaching experience very lively, refreshing and intellectually stimulating. When ready to transition to an independent position, he found the strong focus on teacher-scholar model within the Department of Natural Sciences at UM-Dearborn as the main attractor. The proximity and accessibility to major research one institutes (Wayne State and UM-Ann Arbor) was a bonus.

Education

Johns Hopkins University, School of Medicine, Baltimore, Maryland
Postdoctoral training, Cellular and Molecular Physiology

Wayne State University, School of Medicine, Detroit, Michigan
Ph.D., Biochemistry and Molecular Biology

Wayne State University, College of Science, Detroit, Michigan
M.S., Molecular Biotechnology 

Teaching and Research

Courses Taught

Research

Kondapalli laboratory studies cellular cation regulatory mechanisms that underlie health and disease. Specifically, research focuses on the role of a multifunctional protein NHE9, localized to endosomal membranes within the cells. On one hand, NHE9 is a nanomachine transporting protons out of the endosome in exchange for sodium or potassium ions, thus regulating pH within the endosomal lumen. On the other hand, NHE9’s C-terminal cytoplasmic tail modulates various important cellular signaling pathways via its protein interaction network. Cells use the endosomal system to accomplish crucial functions such as receptor internalization and recycling, exocytosis, and lysosomal degradation. A strict regulation of the endosomal pH is critical to achieve these functions. As a result of both endosomal pH regulation and protein-protein interactions, NHE9 is involved in a multitude of physiological functions. It is therefore unsurprising that NHE9 dysfunction is implicated in many human diseases. For example, loss of NHE9 function is associated with familial autism and attention deficit hyperactivity disorder. Similarly, overexpression of NHE9 underlies a subtype of grade 4 glioblastoma, which is a lethal form of adult brain cancer with no known cure. The overall objective of Kondapalli laboratory is to dissect the cellular mechanisms connecting NHE9 to these diseases with the ultimate goal of developing treatment strategies.

Selected Publications

For a full publication list see: Kalyan C. Kondapalli's Bibliography

Recent Publications

* Indicates UM-Dearborn undergraduate students

  1. Shamroukh, HS*, Lone N*, Akhtar M*, Altayib A*, Sutliff S*, Kassem Z*, Tripathy SK, and Kondapalli KC. The sodium proton exchanger NHE9 regulates phagosome maturation and bactericidal activity in macrophages. Journal of Biological Chemistry. 2022; 10.1016/j.jbc.2022.102150
  1. Pall AE*, Guntur D*, Juratli L*, Bandyopadhyay K and Kondapalli KC. A gain of function paradox: Targeted therapy for glioblastoma associated with abnormal NHE9 expression. Journal of Cellular and Molecular Medicine. 2019; DOI: 10.1111/jcmm.14665
  1. Rawat S, Kondapalli KC, Rodrigues AV and Stemmler TL. Backbone resonance assignments and secondary structure of the apo-Drosophila melanogaster frataxin homolog (Dfh). Biomolecular NMR Assignments. 2019; 13(2):377-381.
  1. Prasad H, Dang DK, Kondapalli KC, Natarajan N, Cebotaru V, Rao R. NHA2 promotes cyst development in an in vitro model of polycystic kidney disease. The Journal of Physiology, 2019; 597(2):499-519.
  1. Gomez Zubieta DM*, Hamood MA*, Beydoun* R, Pall AE* and Kondapalli KC. MicroRNA-135a regulates NHE9 to inhibit proliferation and migration of glioblastoma cells. Cell Communication and Signaling. 2017; 15(1):55.
  1. Beydoun R*, Hamood MA*, Gomez Zubieta DM* and Kondapalli KC. Na+/H+ Exchanger 9 Regulates Iron Mobilization at the Blood Brain Barrier in Response to Iron Starvation. Journal of Biological Chemistry. 2017; 292(10):4293-4301.
  1. Kondapalli KC, Todd Alexander R, Pluznick JL and Rao R. NHA2 is expressed in distal nephron and regulated by dietary sodium. Journal of Physiology and Biochemistry. 2016; 73(2):199-205.
  1. Kondapalli KC, Llongueras JP, Capilla-González V, Prasad H, Hack A, Smith C, Guerrero-Cázares H, Quiñones-Hinojosa A and Rao R. A leak pathway for luminal protons in endosomes drives oncogenic signalling in glioblastoma. Nature Communications. 2015; 6:6289.
  1. Kondapalli KC, Prasad H and Rao R. An inside job: how endosomal Na(+)/H(+) exchangers link to autism and neurological disease. Frontiers in Cellular Neuroscience. 2014; 8:172.
  1. Kondapalli KC, Hack A, Schushan M, Landau M, Ben-Tal N and Rao R. Functional evaluation of autism-associated mutations in NHE9. Nature Communications. 2013;4:2510.

Book Chapters

Bencze KZ, Kondapalli KC, and Stemmler T L. X-Ray Absorption Spectroscopy in Applications of Physical Methods in Inorganic and Bioinorganic Chemistry, pp.513-528. 2007John Wiley & Sons, Ltd, Chichester, UK

Kondapalli KC, Dancis A and Stemmler TL. Molecular Interaction between Frataxin and Ferrochelatase during Heme Assembly, Biochemistry: Synthetic Models and Cellular Systems. 2008. Vol. 1, ACS Publishing, Michael Baldwin and Eric Long, Eds, 17-30